Extraction of cfDNA
For high yield extraction of cfDNA from blood plasma
The importance of blood collection methods in cell-free DNA applications
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​Cell-free DNA (cfDNA) are usually short ~160 bp DNA fragments circulating in the blood plasma of individuals. In oncology and prenatal studies, they offer tremendous potential for non-invasive variant analysis in disease detection, diagnosis and monitoring. Extracting cfDNA from plasma or serum can be challenging due to its low concentration and small fragment size. There is, therefore, a need for an isolation method that is highly efficient at recovering the maximum possible yield of smaller fragment sizes. The yield and concentration of the extracted cfDNA are both critical for improving sensitivity of downstream assays. Maximising the copies of cfDNA which enter your analytical assay are affected by the yield and concentration from the extraction stage of your workflow.
Simple, flexible and scalable
The Bead Xtract cfDNA kit is simple and easy to use and is completely scalable for input volume of plasma between 0.5ml-10ml per extraction. Whats more elution volume can be altered to improve concentration (30-60 μl) even when starting with 10 ml of plasma
Minimal genomic DNA contamination
Optimised extraction buffers further minimise contaminating HMW gDNA which would affect downstream analytical testing. Extracted cfDNA is perfect for qPCR, NGS or dPCR
Automation friendly protocol
Fully automated or manual protocol compatible with most open liquid handlers.
Streamlined workflow
Bead Xtract cfDNA kit enables rapid magnetic separation even when using large volumes. The manual process takes less than 70 minutes with a hands-on time of under 30 minutes
Technical Performance
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cfDNA yield comparison
The high binding capacity of the magnetic particles used in the Bead Xtract cfDNA kit ensures a high DNA yield from plasma samples compared to other leading manufacturers cfDNA extraction kits (Figure 1). It also reduces the amount of particles required to isolate the DNA ensuring that the final elution volume is kept low (30-60 μl) even when starting with 10 ml of plasma. This avoids the need for DNA vacuum concentration steps simplifying the protocol and ensuring the concentration of the final cfDNA sample is high enough for downstream applications.
Figure 2. Reliable and reproducible results
Automated cfDNA extraction using the Bead Xtract cfDNA kit shows expected nucleosomal patterning typical of cfDNA fragment distribution and low gDNA contamination. 4 ml replicates performed on the Hamilton ML STAR and eluted in 50 µl of buffer. Purified DNA was analyzed on the Agilent 4200 Tapestation
Figure 3. Streamlined workflow
​Utilising paramagnetic beads to maximize the binding of short cfDNA fragments. Minimal hands-on time <30 minutes.
​​​Ordering Information​
Part Number Description
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PRE-EXT-020 cfDNA Bead Xtract kit, 20 reactions
PRE-EXT-200 cfDNA Bead Xtract kit, 200 reactions
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